Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012
Comparative Study of Propolis and its Extract on Different Volatile Oil Constituents
Yang Qin, Zhou Juan, Huang Xinxin, Zhang Lin, Zhu Huifen, Yu Shumei, Mao Riwen
(Natural Medicine Engineering Technology Research Center by Supercritical Fluid Extraction in Jiangsu province, Zhenjiang 212009,China)
【Objective】To determine the amount of volatile oil in propolis glue using the following methods: solvent extraction, propolis ethanol extraction and supercritical CO2 extraction. GC-MS was used to determine both the qualitative and quantitative data.
【Result】The content of volatile oil in the propolis was in the following order: supercritical CO2 extraction > ethanol extraction > propolis glue. The content of volatile oil in the propolis extracted by supercritical CO2 was 23.45% higher than the propolis extracted with ethanol. The results of GC-MS
indicated that propolis extracted using supercritical CO2 method recorded a high content of volatile oil (54.8%) in two kinds of common volatile componential extracts which was superior to the propolis extracted with ethanol. More than 1% of the composition of volatile propolis extracted by supercritical CO2 method accounted for 64.7%. This indicated that the volatile oil composition in propolis extracted by supercritical CO2 method was better than the ones extracted with ethanol. Especially terpene; a kind of component found in the propolis extracted by supercritical CO2 was 1.5 times higher than the propolis extracted with ethanol.
【Conclusion】The content of propolis extracts and its volatile oil was highest in the propolis extracted by supercritical CO2. The results indicated that the supercritical CO2 extraction method for extracting propolis and its volatile oil is better than that of ethanol extraction.
Corresponding author, E-mail: firstname.lastname@example.org
23.45%；通过GC-MS分析，两种提取物共有的挥发性成分中，蜂胶超临界C02提取物中含量高的占54.8%，优于蜂胶乙醇提取物；在含量＞1%的挥发性成分中，蜂胶超临界CO2提取物占64.7%，进一步说明蜂胶超临 界CO2提取物挥发性成分优于蜂胶乙醇提取物的挥发性成分。尤其是萜烯类成分，蜂胶超临界CO2提取物中 萜烯类成分含量是蜂胶乙醇提取物中萜烯类成分含量的1.5倍。