J Dent (Tehran), 2013 Jan;10(1):10-5
OBJECTIVE:
Honey has antibacterial activity. The aim of this study was
to evaluate the antibacterial activity of honey on Streptococcus mutans and
Lactobacillus.
MATERIALS AND METHODS:
In this in vitro study, solutions containing 0%, 5%, 10%,
20%, 50% and 100%(w/v) of natural Hamadan honey were prepared. Each blood
(nutrient) agar plate was then filled with dilutions of the honey. The strains
of bacteria were inoculated in blood agar for 24 hours at 37°C and were
adjusted according to the McFarland scale (10×10 cfumcl(-1)). All assays were
repeated 10 times for each of the honey concentrations. Data were analyzed by
non parametric Chi-Square test. Statistical significance was set at α=0.05.
RESULTS:
Significant antibacterial activity was detected for honey on
Streptococcus mutans in concentrations more than 20% and on Lactobacillus in
100% concentration (P < 0.05).
CONCLUSION:
It seems that antibacterial activity of honey could be used
for prevention and reduction of dental caries.
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