Cryo Letters, 2014 September/October;35(5):427-437
The cryopreservation procedures that allow preserving sperm cells have been applied for sperm of many species. A sugar like glucose, fructose and sucrose were frequently used in cryomedia but up to the present pine honey was not used for cryopreservation of sperm cells.
The objective of present study is to investigate the effect of pine honey in various concentrations of 100, 200, 300, 400 and 500 mg/ml solutions on cryopreservation and fertilization ability of spermatozoa of common carp (Cyprinus carpio).MATERIALS AND METHODS: Totally 12.5 % (v/v) Me2SO as a cryoprotectant and 10 % (v/v) egg yolk added all extenders. Pine honey also compared with sugars as glucose, fructose (monosaccharide) and sucrose (disaccharide). Collected semen samples were diluted at the ratio of 1:9 with the extenders. After dilutions, the sperm motility was assessed for each group and then the diluted semen samples were cryopreserved.
The extenders containing 300 mg ml-1 pine honey group showed both highest post thaw motility 75.3 ± 5.1 %, motility duration (s) 47.3 ± 2.5 % and hatching ratio 42.6 ± 4.2 % than other cryopreserved groups (P < 0.05).
Using the pine honey in cryomedia is effective for cryopreservation especially about hatching success of egg fertilized by frozen-thawed sperm of common carp.