Wednesday, December 26, 2012

Honey Has Positive Effects on Metabolism of Patients with Type 1 Diabetes


Metabolic Effects of Honey in Type 1 Diabetes Mellitus: A Randomized Crossover Pilot Study
J Med Food, 2012 Dec 20
The aim of this study was to evaluate the metabolic effects of 12-week honey consumption on patients suffering from type 1 diabetes mellitus (DM).
This was a randomized crossover clinical trial done in the National Institute for Diabetes and Endocrinology, Cairo, Egypt. Twenty patients of both sexes aged 4-18 years with type 1 DM and HbA1C < 10% participated in the study. They were randomized into two equal groups (intervention to control and control to intervention). The dietary intervention was 12-week honey consumption in a dose of 0.5 mL/kg body weight per day.
The main outcome measures were serum glucose, lipids, and C-peptide, and anthropometric measurements. None of participants were lost in follow-up. The intervention resulted in significant decreases in subscapular skin fold thickness, fasting serum glucose, total cholesterol, serum triglycerides, and low-density lipoprotein, and significant increases in fasting C-peptide (FCP) and 2-h postprandial C-peptide (PCP). As possible long-term effects of honey after its withdrawal, statistically significant reductions in midarm circumference, triceps skin fold thickness, SSFT, FSG, 2-h postprandial serum glucose, TG, and HbA1C, and significant increases in FCP and PCP were observed.
This small clinical trial suggests that long-term consumption of honey might have positive effects on the metabolic derangements of type 1 DM.

Tuesday, December 25, 2012

Apimedica: Apitherapy and Heat-Sensitive Therapy Have Synergistic Effect in Treating Ankylosing Spondylitis - 基于热敏点蜂刺治疗强直性脊柱炎的临床对照研究

Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012
Zhenjiang, China
Randomly Controlled Study of Ankylosing Spondylitis Treated by Apitherapy Based on Heat-Sensitive Point
HU Yun , HUANG Rui-cong , WANLAI Si-qi,WANG Yu-ting , RUAN Bo , LI Wan-yao*
ObjectiveTo investigate the clinical effect based on apitherapy on heat-sensitive point. Method 60 cases have been divided into two groups randomly and there were 30 cases in each group. The main syndromes has been recorded in each group.
ResultCompared to the condition before treatment, all the values of main syndrome have improved, and the difference has statistical significance; compared the apitherapy group, the main syndromes in apitherapy based on heat-sensitive point have been more improved and the difference has statistical significance.
ConclusionBoth of apitherapy and heat-sensitive therapy have clinical therapeutic effect, and the united treatment has synergistic effect.
* Corresponding author, E-mailliwanyao@yahoo.com.cn
基于热敏点蜂刺治疗强直性脊柱炎的临床对照研究
1 ,黄瑞 2 ,籁思琪 2 ,钰婷 2 , 3 ,李万瑶 2*
1. 南京中医药大学附属无锡医院,江苏无锡 214001,中国; 2.广州中医药大学针推学院,广东广州510405,中国;3.广东省东莞市中医院,广东东莞 523000,中国)
【目的】探讨基于热敏点蜂刺疗法对强直性脊柱炎的疗效。
【方法】将60 AS 患者分为蜂刺治疗组和 热敏点蜂刺治疗组,每组各30 例。记录两组患者临床主要症状。
【结果】与治疗前相比,两组患者主要症 状有所改善,差异有统计学意义;与蜂刺治疗组相比,热敏点蜂刺治疗组在主要症状、体征改善情况更好,差异有统计学意义。
【结论】蜂刺疗法和热敏灸疗法,两种疗法疗效确切,联合治疗AS 协同作用。
键词: 热敏灸;热敏点;蜂刺;强直性脊柱炎

Monday, December 24, 2012

Apimedica: Bee Venom Can Improve Heart Function - 蜂毒对心衰的治疗作用研究

Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012
Zhenjiang, China
Research on the Therapeutic Effect of Bee Venom on Cardiac Failure
LIU Jie, YANG Wen-chao, MIAO Xiao-qing*
(Bee Science College of Fujian Agriculture and Forestry University, State and Local Joint Engineering Laboratory of Natural Biotoxi, Fuzhou 350002,China )
This study aims to explore the effect and the possible therapeutic mechanism of whole bee venom on cardiac failure, in order to provide the experimental evidence for the clinical application of bee venom anti-heart failure.
Few researches have been done to discover the effect of bee venom in the treatment of heart disease at home and abroad. This experiment observed the therapeutic effect of the venom solution of mice with heart failure, and explored its functioning mechanism by measuring the physiological parameters of the heart.
The results show that bee venom solution can improve the heart function of mice, alleviate the symptoms of cardiac failure. The extent of heart / body weight ratio decreases are: venom dose group (40%) > control group (30%) > captopril group (28%) > venom high-dose bee venom group (14%) > low-dose bee venom group (9%). From the heart rate changes, high-dose bee venom group (319bpm) > captopril group (296 bpm)> control group (256 bpm)> medium-to-low-dose bee venom group (240 bpm). B-type natriuretic peptide (BNP) assay shows that high-dose bee venom group (147.5pg/ml) > control group (130 pg/ml) > low-dose bee vonom group (80 pg/ml) > captopril group (75 pg/ml) > medium-dose bee vonom group (55 pg/ml). In summary, the high-dose group (0.005mg/20g) can increase the heart rate in the short term and is superior to the clinical treatment of heart failure drug, captopril.
*Corresponding author, E-mailmxqsf88@126.com
蜂毒对心衰的治疗作用研究
婕,杨文超,缪晓青*
(福建农林大学蜂学学院,天然生物毒素国家地方联合工程实验室,福州 350002,中国)
本研究旨在探究全蜂毒对心脏衰竭的影响及可能的作用机制,为临床应用蜂毒抗心衰提供实验依据。目前国内外对于蜂毒在心脏疾病方面的治疗效果的研究较少,本实验主要观察蜂毒水溶液对小白鼠心衰的 疗作用,并通过对心脏各项生理指标的测定,探讨其作用机制。结果表明,蜂毒水溶液可改善小鼠心功 能,心衰的症状减轻,心脏/体重比值降低幅度为:蜂毒中剂量组(40%>对照组(30%>卡托普利组(28%> 蜂毒高剂量组(14%>蜂毒低剂量组(9%)。从心率变化情况看,蜂毒低、中剂量组(240 bpm)<对照组256 bpm)<卡托普利组(296 bpm)<蜂毒高剂量组(319bpm)。B 型尿钠肽(BNP)的测定结果显示,蜂毒中剂量组55 pg/ml<卡托普利75 pg/ml<蜂毒低剂量组80 pg/ml对照组130 pg/ml<蜂毒高剂量组 147.5pg/ml综上所述,中剂量组(0.003mg/20g)能有效抑制心率进程并优于临床心衰常用药物卡托普利。
键词: 蜂毒;心衰;心率;联免疫法

Sunday, December 23, 2012

Apimedica: Bee Venom Dose Should Be Adjusted for Each Person, Illness - 蜂针疗法用蜂量的思考


Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012
Zhenjiang, China
Understanding of Bee Dose in Bee Needle Therapy
WANLAI Si-qi, LI Wan-yao*, CAO Xi-jun
(Acupucture and Massage College, Guangzhou University of TCM, Guangzhou 510405, China)
Bee needle therapy was widely welcomed by patients, while there was no standards of bee dose in clinical. For the same disease, some only use a few bees to obtain good results, some use more than hundreds bees. There are great risks and dangers. This article explores this issue.
The dose of bee should be adjusted according to different people, time and illness. A new patient with a small dose is better. Bee Sting quantity generally starts from 1-2, the allergy period maintain 1-3 is appropriate. The dose of patients who had bee needle therapy for a long time may be appropriate to increase, generally maintained at about 10. Severe cases may where appropriate, increase to 30 to 50. Physically weak, sick light, re-treatment should be a small amount of bee needle treatment. Physically strong, the seriously ill who may be appropriate to increase the bee amount. For patients with light disease 1 time a week or two week, they often recover after string 1-3 times. For patients with general acute or severe diseases, once a day, continuing for 2-3 courses or more of treatment. For prevention of recurrence, consider once a month. Small bee dose can’t treat the disease, excessive lead to serious consequences. Strictly control the amount of bee, in order to alleviate the suffering of patients with the most appropriate amount.
Corresponding author, E-mailliwanyao@yahoo.com.cn
针疗法用蜂量的思考
赖思琪,李万瑶*, 曹喜俊
(广州中医药大学,广州 510405,中国)
针疗法在受到患者的广泛欢迎的同时,临床上的用蜂量尚无规范,有的仅用数只蜂治疗取得良好的 效果,有的用上百只治疗同样的疾患,存在极大的风险和危险。本文针对于此探讨了蜂针疗法用量,认为:针量应因人、因时、因病施治。新患者少量为佳,12 只始,过敏期内维持13 为宜;久蜂可适当加 量,一般维持在10 只左右,严重者可酌情加大至3050 只。体虚、病轻、复针者宜少量蜂针治疗,体实、病重者可适当增加蜂量。病情轻浅者可以每周或隔周1 次,13 次可愈;急性病、严重病者需每日1 次,持23 疗程,或更久;预防复发可1 1 次。小蜂量难以愈病,过量易导致严重后果,要严格把握好
针的用量,以期用最合适的量缓解患者的痛苦。

Saturday, December 22, 2012

Apimedica: Integrated Bee Venom Therapy Helps Treat Multiple Sclerosis - 多发性硬化症之中西医结合蜂疗疗效

Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012
Zhenjiang, China
Integrated Apitherapy Effects on Multiple Sclerosis
Siu-Wan Ip1*CHEN Ching-yi2
1. Graduate School of Nutrition, China Medical University, Taizhong 40402, Taiwan China; 2. Asia Pacific Apitherapy Association, Zhanghua 50078, Taiwan China
IntroductionMultiple sclerosis (MS) is a common disease in North America and Europe without effective medical treatment. Apitherapy is an effective treatment in Multiple Sclerosis patients reported in recent years. However, most MS patients missed their golden treatment periods because of allergic reactions after high dose of BVT treatments. 
PurposeThe purpose of this study is to investigate the effect of integrated Apitherapy (Bee venom Treatment) on Multiple Sclerosis patient. 
MethodA 48-year-old female was suffering from blurred vision and weakness of limbs since 2000. Patient was admitted to Taiwan Chang-Hua Christian Hospital because of weakness of limbs and blurred visions. Patient developed shortness of breath and headache later. Because of lacking of improvement,she was transferred to National Taiwan University Hospital (NTUH) later. CT scan performed in NTUH revealed 13 pathological lesions were found in her brain. The diagnosis of Multiple Sclerosis was made after the CT scanning and abnormal laboratory studies of serum and CSF. She was admitted to NTUH for medical treatments for two years. However, there was no obvious improvements after two years medical treatment in NTUH. She visited our Apitherapy clinic on September 5,2002. She was carried to our Apitherapy Clinic by her family members to receive Bee Venom Therapy (BVT) three times weekly. Blood examinations of serum Immunoglobulins (IgG, IgE) were performed regularly to monitor the dosages of Apitherapy. We adjust the BVT dose to maintain the serum IgG level above 1000 mg/dl during the whole BVT course. We lowered the BVT dose or stopped the BVT treatment if patient developed allergic reactions after high BVT dosing treatment. Totally 3098 bees venom were used in four months of BVT treatments. 
ResultAfter received four months of BVT treatment, she started walking on her own on January 15,203. The blurred visions, weakness of limbs and shortness of breath were improved. 
DiscussionMultiple Sclerosis is a common disease in North America and Europe. There was no effective medical treatment up to present time. Apitherapy was reported to be effective in early phase of Multiple Sclerosis patients. However, most Multiple Sclerosis patients stopped BVT treatment because of developed allergic reactions after high dose of BVT. However, we shall miss the golden treatment periods if we stopped the BVT too long. And in North America and Europe, family members will care about the allergic reactions after BVT. Laboratory examinations of serum Immulogublins regularly provide a therapy guide lines in adjusting the dosing of BVT. The combinations of Medical Nutrition Therapy (MNT) during BVT will decrease the risks of allergic reactions and enhance the effects of BVT. High proteins diets may enhance the serum levels of IgG. And avoiding seafood may decrease the IgE level and decrease the risk of allergic reactions.
ConclusionIntegrated Apitherapy monitoring with Immunoglobins (IgG, IgE) during the whole course of Apitherapy can increase the effect of bee venom treatment.
Key words: multiple sclerosis (MS); bee venom therapy (BVT)
______________________________________________
*Corresponding author,E-mailsiuwanye@gmail.com
发性硬化症之中西医结合蜂疗疗效
叶兆云 1*,陈清一2
(1.中国医药大学营养研究所,台中40402,台湾 中国;2.() 亚太蜂针研究会 ,彰化 50078, 台湾 中国)
【前言】多发性硬化症(MS)为北美及欧洲常见之疾病,唯目前仍有效疗法。近年求,很多医学报告蜂针 疗法对多发性硬化症有疗效。但是很多M.S.病人愿接受高剂量的蜂针疗法后过敏,而中止B.V.T,固搞 失治M.S.的黄金时期。
【目的】本研究计划为探讨中西医结合蜂针疗法对多发性硬化症之疗效.
【方法】病者庄XX,48 岁主妇,2000 年开始出现视力减退,失去四肢活动能力。病者首先住入台湾彰化 基督教医院。并出现呼吸急促及头痛等症状。由于无明显疗效,病者转院到台北市的国立台湾大学附设医院 继续治疗.计算机断层扫描(CT scan)检查发现大脑有13 个病灶。血液及CS 血液检查均符合多发性硬化症之 诊断。固此病者继续台大医院之治疗。但在台大医院治疗两年后,病者并无明显的病程进步。因此病者于2002 9 5 日到访本蜂疗诊所。那时候,病者是由家属背着来本诊所就医。她每周回诊三次,接受蜂针治疗。病者定期接受抽血检查免疫球蛋白(IgEIgG检验以调整蜂针之剂量。我们在蜂针疗法全程调整IgG 数在1000mg/dl 以上。若病者出现过敏现象,们会降低BVT 剂量,暂停BVT。病者在四个月内会全部接 受了3098 只蜂针治疗。
结果】经过四个月蜂针治疗后,病者于2003 1 15 日后能自行走路。视力减退,呼吸急促及四肢 无力均有明显改善。
讨论】多发性硬化症(M.S.)为欧美常见之疾病,但到目前为止现代医学并无有疗法。据近年医学报 ,针疗法对早期的M.S.疗效。但很多病者在接受高剂量蜂针疗法后,现过敏现象,以至于蜂疗被迫 中止,因而错失治疗M.S.的黄金时期。但在欧美国家,病者家属对蜂针之过敏现象十分关注。因此,针期 ,定期抽血,实验室检查免疫球蛋白指数,调整蜂针剂量,可作为蜂疗的剂量指导原则。另外,针期间, 结合现代医学营养疗法(MNT),可增强蜂针疗法之疗效及降低过敏反应。高蛋白膳食疗法可提高血清IgG 指教。避免摄食海产食物可降低IgE 及减少蜂疗过敏反应。
结论】中西医结合蜂针疗法,以血中免疫球蛋白(IgG,IgE)指数指导调节蜂针剂量,可增强蜂疗疗效。键词:发性硬化症(M.S.);蜂针疗法 (BVT)

Friday, December 21, 2012

Apimedica: Bee Pollen is an Effective Natural Anti-Oxidant - 玉米蜂花粉油脂抗氧化性研究及成分分析

Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012
Zhenjiang, China
Antioxidant Activity and Composition Analysis of Corn Bee Pollen Oil
SUN Li-ping*, XU Xiang, HE Wei
(Bee Research Institution, The Chinese Academy of Agricultural Sciences, Beijing 100093China)
The capacity of bee pollen oil is evaluated by these three indicators: ABTS, DPPH radical scavenging and the phosphomolybdate complex. The content of carotenoid is determined by spectrophotometry, and the phospholipid composition is quantitatively analyzed by HPLC. The results show that corn bee pollen oils have strong scavenging ability on the ABTS, DPPH free radicals, and the IC50 values are 0.26±0.01mg/mL2.20±0.05mg/mL respectively. A strong antioxidant capacity has also identified by the Phosphomolybdate complex method. Corn Bee Pollen, in which carotenoid content is 16.21 ± 0.84 mg/100g and the total phospholipid content is 2.98 ± 0.02g/100g, contains five phospholipid composition, namely, PI, PS, PE, PC, LPC. The proportion of carotenoids and total phospholipid in grease is 0.21% and 38.11%. Finally, the conclusion is drawn that corn bee pollen contains a variety of fat-soluble antioxidant active ingredients, can supply hydrogen or electrons, and is an effective natural anti-oxidant resources.
Key words: corn and bee pollen; antioxidant; fat-soluble components; content;
___________________________________________
*Corresponding author,E-mailcaasum@126com.cn
玉米蜂花粉油脂抗氧化性研究及成分分析
孙丽萍*,徐 响,何
(中国农业科学院蜜蜂研究所,北京 100093,中国)
ABTSDPPH 自由基清除和磷钼络合物法三个指标评价蜂花粉油脂的抗氧化能力,通过分光光度法 测定类胡萝卜素含量,HPLC 法定量分析其中的磷脂成分。结果表明,玉米蜂花粉油脂对ABTSDPPH 由基有较强的清除能力,IC50 值分别为0.26±0.01mg/mL2.20±0.05mg/mL,在磷钼络合物法中也表现出较 强的抗氧化能力。玉米蜂花粉中类胡萝卜素含量为16.21±0.84 mg/100g 花粉,总磷脂含量为2.98± 0.02g/100g 花粉,含有PIPSPEPCLPC 五种磷脂成分。类胡萝卜素和总磷脂含量占油脂的比例为0.21% 38.11%结论:玉米蜂花粉含有多种脂溶性抗氧化活性成分,有很好的供应氢或电子的能力,是一种有 效的天然抗氧化剂资源。
键词:玉米蜂花粉;抗氧化;脂溶性成分;含量

Brazilian Red Propolis Inhibits Cancer Cell Growth


Chemical Characterization, Antioxidant and Cytotoxic Activities of Brazilian Red Propolis
Food and Chemical Toxicology, Volume 52, February 2013, Pages 137–142
Propolis is known for a long time for its health benefits and biological activities. Here, the red variety from the northeast of Brazil was chemically analyzed and extracts were investigated regarding their antioxidant and antitumor activity.
Hydroalcoholic extracts, obtained from the red propolis, revealed polyphenol content, 2,2-diphenyl-1-picrylhydrazyl scavenging potential and enzymatic activities for catalase-like and superoxide dismutase-like. Cytotoxic activity was evaluated for human laryngeal epidermoid carcinoma cell (Hep-2), human cervical adenocarcinoma (HeLa) and human normal epithelial embryonic kidney (Hek-293). Survival analysis for non-tumor cell line showed greater IC50 compared to tumor cell lines, suggesting an increased sensitivity that may correlate with the higher proliferative index of the tumor vs. normal cells.
Our results indicate that the Brazilian red propolis is capable of inhibiting cancer cell growth and constitutes an excellent source of antioxidant and antitumor natural agent.