Propolis Inhibits the Proliferation of Human Leukaemia HL-60 Cells by Inducing Apoptosis Through the Mitochondrial Pathway
Natural Product Research, Volume 24, Issue 4 March 2010 , pages 375-386
Propolis, a natural product derived from plant resins collected by honeybees, has been reported to exert a wide spectrum of biological functions. This research aimed at investigating the effect of propolis on the proliferation of human leukaemia HL-60 cells and whether propolis might induce apoptosis in HL-60 cells.
The results showed dose- and time-dependent decreases in the proliferation of HL-60 cells treated with propolis (above 3 µg mL-1 of propolis). Further studies revealed that the anti-proliferative effects of propolis were caused by inducing apoptosis. Agarose electrophoresis of genomic DNA of HL-60 cells treated with propolis showed the ladder pattern typical for apoptotic cells. Propolis induced the activation of caspase-3 and cleavage of poly (ADP-ribose) polymerase in HL-60 cells. Propolis also induced the release of cytochrome c from mitochondria to cytosol.
Taken together, these findings demonstrate that the inhibitory effect of propolis on HL-60 cell proliferation is caused by inducing apoptosis through the mitochondrial pathway.