Wednesday, December 15, 2010

New Method to Measure Antioxidant Capacity of Propolis

Rapid and Effective Evaluation of the Antioxidant Capacity of Propolis Extracts Using DPPH Bleaching Kinetic Profiles; FT-IR and UV-vis Spectroscopic Data
Journal of Food Composition and Analysis, Article in Press

An informative and effective measure of antioxidant capacity based on DPPH (2, 2-diphenyl-1-picrylhydrazyl) bleaching kinetic profiles has been developed using principal component analysis (PCA).

The activity score and a related parameter, called quercetin factor (QF), were used to estimate antioxidant capacity for 39 propolis extracts based on the first principal component (which explains 98% of the total variance). Determination of the QF parameter requires less time and reagents than previous DPPH-based antioxidant capacity parameters, but does require additional equipment. Additionally, UV-vis and FT-IR spectroscopic features of propolis extracts have been identified, which have been correlated to antioxidant capacity, and offer a spectroscopic and reagent-less rapid evaluation method of the antioxidant activity of biological samples.

Together the QF scores based on DPPH bleaching and FT-IR and UV-vis spectra are analyzed in terms of antioxidant capacity, floral origin, and geographic location.

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