Antimicrobial Effect of Conventional Root Canal Medicaments
vs Propolis against Enterococcus faecalis, Staphylococcus aureus and Candida
albicans
J Contemp Dent Pract, 2012 May 1;13(3):305-9.
Aim: To evaluate and compare antimicrobial effect of various
root canal medicaments against Enterococcus faecalis, Staphylococcus aureus and
Candida albicans.
Materials and methods: Six root canal medicaments: 2% sodium
hypochlorite (NaOCl), 2% chlorhexidine (CHX) Calcium hydroxide (Ca(OH)2), EDTA,
MTAD and propolis and three microorganisms: Staphylococcus aureus, Enterococcus
faecalis and Candida albicans were used. These strains were inoculated in brain
heart infusion (BHI) and incubated at 37 degrees C for 24 hours. For the agar
diffusion test (ADT), petri plates with 20 ml of BHI agar were inoculated with
0.1 ml of the microbial suspensions, using sterile swabs that were spread on
the medium, obtaining growth injunction. Paper disks were immersed in the experimental
solutions for 1 minute. Subsequently, four papers disks containing one of the
substances were placed on the BHI agar surface in each agar plate. The plates
were incubated at 37°C for 48 hours. The diameter of microbial inhibition was
measured around the papers disks containing the substances. One way ANOVA
followed by Tukey's post-hoc test were used. p-value >0.05 was considered
statistically significant.
Results: Propolis and other irrigants were found to be
effective on C. albicans, S. aureus and E. faecalis. CHX and MTAD were found to
be most effective amongst all the materials tested followed by propolis.
Conclusion: Propolis showed antimicrobial activity against
E. faecalis, S. aureus, C. albicans. It appears that propolis is an effective intracanal
irrigant in eradicating E. faecalis and C. albicans.
Clinical significance:
Propolis is an effective intracanal irrigant in eradicating E. faecalis and C.
albicans. It could be used as an alternative intracanal medicament.
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