Effect of Croatian Propolis on Diabetic Nephropathy and Liver
Toxicity in Mice
BMC Complementary and Alternative Medicine 2012, Published:
6 August 2012
Background
In the present study, we examined the antioxidant effect of
water soluble derivative of propolis (WSDP) and ethanolic (EEP) extract of
propolis on renal and liver function in alloxan-induced diabetic mice. In
addition, we examined whether different extract of propolis could prevent
diabetic nephropathy and liver toxicity by inhibiting lipid peroxidation in
vivo.
Methods
Diabetes was induced in Swiss albino mice with a single
intravenous injection of alloxan (75 mg kg-1). Two days after alloxan injection,
propolis preparations (50 mg kg-1 per day) were given intraperitoneally for 7
days in diabetic mice. Survival analysis and body weights as well as
hematological and biochemical parameters were measured. The renal and liver
oxidative stress marker malonaldehyde levels and histopathological changes were
monitored in the liver and kidney of treated and control mice.
Results
Administration of propolis to diabetic mice resulted in a
significant increase of body weight, haematological and immunological parameters
of blood as well as 100% survival of diabetic mice. Alloxan-injected mice
showed a marked increase in oxidative stress in liver and kidney homogenate, as
determined by lipid peroxidation. Histopathological observation of the liver
sections of alloxan-induced diabetic mice showed several lesions including
cellular vacuolization, cytoplasmic eosinophilia and lymphocyte infiltrations,
but with individual variability.Treatment of diabetic mice with propolis
extracts results in decreased number of vacuolized cells and degree of
vacuolization; propolis treatment improve the impairment of fatty acid
metabolism in diabetes. Renal histology showed corpuscular, tubular and
interstitial changes in alloxan-induced diabetic mice. Test components did not
improve renal histopathology in diabetic mice.
Conclusions
Propolis preparations are able to attenuate diabetic
hepatorenal damage, probably through its anti-oxidative action and its
detoxification proccess as well as the potential to minimize the deleterious effects
of free radicals on tissue. The protective role of propolis against the ROS
induced damages in diabetic mice gives a hope that they may have similar
protective action in humans.
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