Assessment of Molecular Events During in vitro Re-Epithelialization
Under Honey-Alginate Matrix Ambience
Mater Sci Eng C Mater Biol Appl, 2013 Aug 1;33(6):3418-3425
Re-epithelialization is one of the most important stages of
cutaneous regeneration and its success requires supportive micro-ambience which
may be provided with suitable bio-matrix. Biocompatibility and efficacy of such
bio-matrix in re-epithelialization could be explored by multimodal analysis of
structural and functional attributes of in vitro wound healing model including
evaluation of prime molecular expressions of the epithelial cells during
repair.
Present study examines the influence of honey-alginate and
alginate matrices on re-epithelialization in keratinocyte (HaCaT) population in
a 2-D wound model.
Cellular viability, proliferation and cell-cell adhesion
status were assessed during wound closure using live/dead cell assay and by
evaluating expressions of Ki67, p63 and E-cadherin along-with % change in
cellular electrical impedance. Efficacy of honey-alginate matrix in comparison
to only alginate one was demonstrated by a quicker reduction in wound gap,
improved cellular viability, enhanced expressions of Ki67, p63 and its isoforms
(TAp63, ΔNp63) as well as E-cadherin.
Faster restoration of electrical attribute (% of impedance
change) after wounding also indicated better impact of honey-alginate matrix in
re-epithelialization.
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