Biomed Sci Instrum, 2013 Apr 5;49:101-108
Lung cancer is a one of the most prevalent and deadly
cancers in United States. Experimental evidence support that cancer cells do
exhibit higher glycolytic rates than normal cells. To exploit this unique
cancer-dependent ATP generation phenomenon, we hypothesize that exposure of
cancer cells to organic inhibitors of glycolysis would negatively impact their
survival and alter their growth and viability resulting from the vast decrease
in their essential glycolytic ATP production; no negative consequences will be
seen on normal lung cells.
The human lung fibroblast cell line MRC-5 and the human lung
alveolar epithelial cancer cell line A549 were used in this study as models for
normal lung and lung cancer respectively. Using standard methods, both cell
lines were maintained and exposed to honey and D-glucose reagents at
concentration levels ranging from 31.3-2,000 µg/ml in 96 well plates in
quadruplets and experiments repeated at least three times using MTT (3-(4,
5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide), and cell counting (T4
Cellometer; automated cell counting system) assays as well as phase-contrast
photo-imaging.
Our results indicate that exposure of both cell lines to
these organics lead to concentration dependent cell destruction/cell survival
depending on the cell line exposed. Honey and D-glucose showed statistically
significant (p < 0.05) differential negative effects on the A549 line in
comparison to its unexposed control as well as to their effects on the MRC-5
cell line.
These findings show a promising role for honey and D-glucose
as biotherapeutic metabolites of interest for selective management of cancerous
cells.
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