Melittin suppresses EGF-induced cell motility and invasion
by inhibiting PI3K/Akt/mTOR signaling pathway in breast cancer cells
Food Chem Toxicol, 2014 Mar 24. pii: S0278-6915(14)00151-3
Bee venom is a natural compound produced by the honey bee
(Apis mellifera), and has been reported as having the biological and
pharmacological activities, including anti-bacterial, anti-viral and
anti-inflammation. In the present study, the inhibitory effects of bee venom
and its major peptide components on the tumor invasion were demonstrated. It was
confirmed the inhibitory effects of bee venom, melittin, and apamin on the
EGF-induced invasion of breast cancer cells. Transwell invasion and
wound-healing assays showed that bee venom and melittin significantly inhibits
the EGF-induced invasion and migration of breast cancer cells. Also, bee venom
and melittin reduced the EGF-stimulated F-actin reorganization at the leading
edge, but apamin did not affect. Particularly, melittin inhibited the
EGF-induced MMP-9 expression via blocking the NF-κB and PI3K/Akt/mTOR pathway.
In addition, melittin significantly suppressed the EGF-induced FAK
phosphorylation through inhibition of mTOR/p70S6K/4E-BP1 pathway. These results
suggest that inhibitory effects of melittin on breast cancer cell motility and
migration may be related to the inhibition of mTOR pathway.
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