Caffeic acid phenethyl ester, a major component of propolis,
suppresses high fat diet-induced obesity through inhibiting adipogenesis at
mitotic clonal expansion stage
J Agric Food Chem, 2014 Mar 10
In the present study, we aimed to investigate anti-obesity
effect of CAPE in vivo, and the mechanism by which CAPE regulates body weight in
vitro. To confirm anti-obesity effect of CAPE in vivo, mice were fed with a
high fat diet (HFD) with different concentrations of CAPE for 5 weeks. CAPE
significantly reduced body weight gain and epididymal fat mass in obese mice
fed a HFD.
In accordance with in vivo results, Oil red O staining
results showed that CAPE significantly suppressed MDI-induced adipogenesis of
3T3-L1 preadipocytes. FACS analysis results showed that CAPE delayed
MDI-stimulated cell cycle progression, thereby contributing to inhibit mitotic
clonal expansion (MCE) which is prerequisite step for adipogenesis. Also, CAPE
regulated expression of cyclin D1 and phosphorylation of ERK and Akt which are
upstream of cyclin D1.
These results suggest that CAPE exerts anti-obesity effect
in vivo, presumably through inhibiting adipogenesis at early stage of
adipogenesis
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