Detection of C. Botulinum Types in Honey by mPCR
J Food Sci, 2014 Mar 12. doi: 10.1111/1750-3841.12402
The aim of this study was to determine the prevalence of
Clostridium botulinum in honey samples using conventional methods and multiplex
PCR (mPCR). A total number of 150 honey samples were randomly collected from
apiaries, retail shops, weekly open bazaars, and supermarkets in Samsun,
Turkey. Of 150 honey samples, 4 (2.6%) were positive for the botulinum
neurotoxin gene by mPCR analysis. A total of 4 C. botulinum isolates were
obtained from the mPCR positive samples, of which 3 were type A and 1 was type
B. No samples were positive regarding the type E and type F neurotoxin genes.
This is the first report of type A and type B spores of C. botulinum being
detected and isolated in Turkey. This study revealed that some honey samples
may present a potential hazard for food borne and infant botulism.
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