Anti-HIV-1 Activity of Eight Monofloral Iranian Honey Types
PLoS One. 2014; 9(10): e108195
Monofloral Iranian honeys from eight floral sources were
analyzed to determine their anti-HIV-1 activities as well as their effects on
lymphocyte proliferation. The Peripheral Blood Mononuclear Cells (PBMCs) used
in this study were prepared from five healthy volunteers who were seronegative
for HIV, HCV, HBV and TB.
The anti-HIV-1 activity of eight different honeys was
performed by quantitative polymerase chain reaction (PCR) assay and high pure
viral nucleic acid kit. The results demonstrated that monofloral honeys from
Petro selinum sativum, Nigella sativa, Citrus sinensis, Zataria multiflora,
Citrus aurantium and Zizyphus mauritiana flowers had potent anti-HIV-1 activity
with half maximal effective concentration (EC50) values of 37.5, 88, 70, 88,
105 and 5 µg/ml respectively. However, monofloral Iranian honeys from
Astragalus gummifer and Chamaemelum nobile flowers had weak anti-HIV-1
activity. The frequency and intensity of CD4 expression on PBMCs increased in
the presence of all honey types. CD19 marker were also increased after the
treatment with monofloral honeys from Z.multiflora and N. sativa.
The anti-HIV-1 agent in monofloral honeys from P.sativum, N.
sativa, Z. multiflora and Z. mauritiana flowers was detected by spectroscopic
analysis as methylglyoxal. Time of drug addition studies demonstrated that the
inhibitory effect of methylglyoxal is higher on the late stage of HIV-1
infection. The result demonstrated that methylglyoxal isolated from monofloral
honey types is a good candidate for preclinical evaluation of anti-HIV-1
therapies.
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