Histone deacetylase inhibitory effect of Brazilian propolis
and its association with the antitumor effect in Neuro2a cells
Food Sci Nutr. 2014 Sep;2(5):565-570
Propolis is a resinous product produced by honey bees and is
known to have antitumor functions. On the other hand, histone deacetylase
(Hdac) inhibitors have recently attracted attention for their antitumor
effects. In this study, we examined whether Brazilian green propolis has an
Hdac inhibitory activity and its contribution on antitumor effects. By in vitro
Hdac activity assay, Brazilian propolis extract (BPE) significantly inhibited
the enzyme activity. Actually, BPE treatment increased the intracellular
histone acetylation in Neuro2a cells. Regarding antitumor effect in Neuro2a
cells, BPE treatment significantly decreased cell viability. An Hdac activator
theophylline significantly attenuated the effect. Then, we analyzed whether the
decreasing effect on cell number was caused by cell death or growth
retardation. By live/dead cell staining, BPE treatment significantly increased
the dead cell number. By cell cycle analysis, BPE treatment retarded cell cycle
at the M-phase. Both of these cellular effects were suppressed by addition of
theophylline. These data indicate that BPE induced both cell death and growth
retardation via Hdac inhibitory activity. We demonstrated that Brazilian
propolis bears regulatory functions on histone acetylation via Hdac inhibition,
and the effect contributes antitumor functions. Our data suggest that intake of
Brazilian propolis shows preventing effects against cancer.
No comments:
Post a Comment