Sunday, June 24, 2007

Royal Jelly Component Involved in Synthesis of Molecule that Stimulates Immune Response

Evidence for New β1-3 Galactosyltransferase Activity Involved in Biosynthesis of Unusual N-Glycan Harboring T-Antigen in Apis Mellifera
Bioscience, Biotechnology, and Biochemistry, Vol. 71 (2007) , No. 4 pp.1111-1114

In a previous study (Y. Kimura et al., Biosci. Biotechnol. Biochem., 70, 2583–2587, 2006), we found that new complex type N-glycans harboring Thomsen-Friedenreich antigen (Galβ1-3GalNAc) unit occur on royal jelly glycoproteins, suggesting the involvement of a new β1-3galactosyltransferase in the synthesis of the unusual complex type N-glycans. So far, such β1-3galactosyltransferase activity, which can transfer galactosyl residues with the β1-3 linkage to β1-4 GalNAc residues in N-glycan, has not been found among any eucaryotic cells. But using GalNAc2GlcNAc2Man3GlcNAc2-PA as acceptor N-glycan, we detected the β1-3 galactosyltransferase activity in membrane fraction prepared from honeybee cephalic portions. This result indicates that honeybee expresses a unique β1-3 galactosyltransferase involved in biosynthesis of the unusual N-glycan containing a tumor related antigen in the hypopharyngeal gland.

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