Wednesday, April 08, 2015

Honey dilution impact on in vitro wound healing: Normoxic and hypoxic condition

Wound Repair and Regeneration

Accepted Article (Accepted, unedited articles published online and citable. The final edited and typeset version of record will appear in future.)

Honey is known as a popular healing agent against tropical infections and wounds. However, the effects of honey dilutions on keratinocyte (HaCaT) wound healing under hypoxic condition is still not explored.

In this study, we examined whether honey dilution have wound healing potential under hypoxic stress. The anti-oxidant potential and healing efficacy of honey dilution on in vitro wound of human epidermal keratinocyte (HaCaT cells) under hypoxia (3% O2) and normoxia is explored by NBT assay. The cell survival % quantified by MTT assay to select 4 honey dilutions like 10, 1, 0.1 and 0.01 v/v % and the changes in cellular function was observed microscopically. Further, the cell proliferation, migration, cell-cell adhesion and relevant gene expression were studied by flow-cytometry, migration/scratch assay, immuno- cytochemistry and RT-PCR respectively. The expression pattern of cardinal molecular features viz. E-cadherin, cytoskeletal protein F-actin, p63 and hypoxia marker Hif 1 α were examined.

Honey dilution in 0.1% v/v combat wound healing limitations in vitro under normoxia and hypoxia (3%). Its wound healing potential was quantified by immuno-cytochemistry and real-time PCR for the associated molecular features that were responsible for cell proliferation and migration. Our data showed that honey dilution can be effective in hypoxic wound healing. Additionally, it reduced superoxide generation and supplied favorable bio-ambience for cell proliferation, migration and differentiation during hypoxic wound healing. These findings may reveal the importance of honey as an alternative and cost effective therapeutic natural product for wound healing in hypoxic condition. This article is protected by copyright. All rights reserved.

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