Research on the Influence of Api-Diet on Seric Proteins Profile in Acetaminofen Experimental Induced Hepatopathy in Wistar Rats
Analele Societatii Nationale de Biologie Celulara; 2009, Vol. 14 Issue 1, p117-123
The aim of this experiment was to study the api-diet influence on seric proteins profile (evaluated by electrophoresis) in rats with acetaminophen experimentally-induced hepatopathy.
In order to reduce factors that precipitate the progression of hepatic lesions, we have administered api-therapy products. Substances: apitherapic products - Apiregya, ApiImunomod, ApiImunostim got from “Stupina SRL” (office and laboratories in the Balanesti parish, Gorj county, Romania, phone 0253.270.221).
Animals were manipulated under general anesthesia with thiopental. The levels of investigated parameters were measured using an automatic analyzer (Aeroset, Abbott) and commercial kits (Abbott, USA). 60 white Wistar rats was used, equally divided into six groups: the control group - standard nourishment (group I); api-diet control group (group II); api-diet and royal jelly (RJ) control lot (group III); acetaminophen group (group IV); api-diet and acetaminophen group (group V); acetaminophen, api-diet , and RJ lot (group VI).
After that, a number of 30 subjects were kept for study and research. Animals were kept in normal light and temperature conditions, water and food being provided ad libidum. Toxic hepatopathy was experimentally induced by acetaminophen administered by intra-gastric gavage (hydrous solution, 1g/kg/day, for 2 weeks). Animals were manipulated under general anesthesia with Thiopental.
In group V, api-diet and acetaminophen co-administration determined a statistically significant increase of serum albimine level compared to: i) group I (standard nourishment control group) (30.42±1.27g/L versus 39.51±2.91g/L, p<0.0001); ii) api-diet control group (group II) (4.49±0.9g/L versus 39.51±2.91g/L, p<0.0001). In group VI, acetaminophen, api-diet, and RJ co-administration determined a statistically significant decrease of total globulins compared to: i) group I (standard nourishment control group) (69.57±1.27g/L versus 61.7±5.07g/L, p<0.0001); ii) group IV (acetaminophen) (95.51±0.9g/L versus 61.7±5.07g/L, p<0.0001). In group VI, acetaminophen, api-diet , and RJ co-administration determined a statistically significant decrease of γ-globulins compared to acetaminophen group (group IV) (9.83±1.2g/L versus 9.7±0.43g/L). Authors recommend apitherapy with Apiregya, ApiImunostim, and ApiImunomod in hepatic affections, but only taken into consideration the serum proteins electrophoresis profile. Also, treatment should be modulated according to the evolution of the above-mentioned parameters.