Effect of neovestitol–vestitol containing Brazilian red
propolis on accumulation of biofilm in vitro and development of dental caries
in vivo
Biofouling: The Journal of Bioadhesion and Biofilm Research,
Published online: 07 Oct 2013
The present study examined the influences of the
neovestitol–vestitol (NV) containing fraction isolated from Brazilian red
propolis on the development of biofilm and expression of virulence factors by
Streptococcus mutans using saliva-coated surfaces of hydroxyapatite. In
addition, NV was tested in a rodent model of dental caries to assess its
potential effectiveness in vivo.
Topical applications of NV (800 μg ml−1) significantly
impaired the accumulation of biofilms of S. mutans by largely disrupting the synthesis
of glucosyltransferase-derived exopolysaccharides and the expression of genes
associated with the adaptive stress response, such as copYAZ and sloA. Of even
greater impact, NV was as effective as fluoride (positive control) in reducing
the development of carious lesions in vivo.
NV is a promising natural anti-biofilm agent that targets
essential virulence traits in S. mutans, which are associated with the
formation of cariogenic biofilm and the subsequent onset of dental caries
disease.
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