In vitro activity of natural honey alone and in combination
with curcuma starch against Rhodotorula mucilaginosa in correlation with
bioactive compounds and diastase activity
Asian Pac J Trop Biomed, 2013 Oct;3(10):816-21
OBJECTIVE:
To evaluate the in vitro activity and synergism of the
combinations of natural honey and curcuma starch against Rhodotorula
mucilaginosa in correlation with total phenolic, flavonoid contents, and
diastase activity.
METHODS:
The Folin-Ciocalteu test was used to determine the total
polyphenols content and the flavonoid content was analyzed using by the
aluminum chloride method. The antifungal activity of the natural honey, determined
by an agar well diffusion assay and agar incorporation method.
RESULTS:
Total phenolic content varied from (63.930.11) to
(95.366.08) mg GAE/100 g honey as gallic acid equivalent. Total flavonoids
content varied from (5.41±0.04) to (9.94±0.54) mg CE/100 g. Diastase activity
values were between (7.3±2.8) and (26±2.8). The zone inhibition diameter for
the six honey samples without starch ranged between 6 and 20 mm. When starch
was mixed with honey and then added to well, a zone inhibition increase
diameter 7 and 21 mm. The percentage increase was noticed with each variety and
it ranged between 5% and 62.5%. The minimal inhibitory concentrations for the
six varieties of honey without starch against Rhodotorula mucilaginosa ranged
between 28% and 36% (v/v). When starch was incubated with honey and then added
to media, a minimal inhibitory concentration drop has been noticed with each
variety. It ranged between 6.66 % and 20% (w/v). No significant correlation was
established between diastase activity and bioactive compounds.
CONCLUSIONS:
The mixture of curcuma starch and honey could lead to the
development of new combination antibiotics against Rhodotorula infections.
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