Green Propolis Displays Better Antimicrobial and Antioxidant Activities

Artepillin C and phenolic compounds responsible for antimicrobial and antioxidant activity of green propolis and Baccharis dracunculifolia DC

Aims

This study investigates the antimicrobial activity in Staphylococcus aureus isolates (MSSA – Methicillin Sensitive Staphylococcus aureus and MRSA – Methicillin Resistant Staphylococcus aureus) and antioxidant activity of green propolis, Baccharis dracunculifolia DC extracts and Artepillin C™.


Methods and Results

The amount of Artepillin C in different extracts was determined by HPLC analysis. Minimum Inhibitory Concentrations 90 (MIC90) was determined using 40 isolates of Staphylococcus aureus inoculated in Müeller-Hinton agar culture medium containing the green-propolis and Baccharis dracunculifolia DC extracts. PVEE (green propolis ethanolic extract) and BDEH (Baccharis dracunculifolia hexanic extract) showed the greatest antimicrobial activity with MIC90 values of 246.3 and 295.5 μg/mL, respectively. Green propolis ethanolic and hexanic extracts (PVEE and PVEH respectively) showed the greatest antioxidant activity assessed by DPPH (1,1-diphenyl-2-picrylhydrazylradical) with IC50 values of 13.09 and 95.86 μg/mL, respectively.

Conclusions

Green propolis ethanolic is displays better antimicrobial and antioxidant activities compared to other extracts. These activities may be related to the presence of Artepillin C in synergism with the other constituents of the extracts.

Significance and impact of the study

In this study, the antimicrobial activity of the extracts of green propolis and Baccharis dracunculifolia DC demonstrated in MRSA an MSSA clinical isolates indicated that they can be important tools to treat infections caused by these bacteria.