Tuesday, January 15, 2013

Apimedica: Saudi Arabian Propolis Shows Antibiotic Properties -沙特阿拉伯阿尔巴哈省蜂胶对人类耐药性病原菌体外抗菌活性研究

Apimondia Apimedica-Apiquality International Forum
Oct. 22-25, 2012
Zhenjiang, China
An in vitro Study on Antimicrobial Activity of Propolis from Al-Baha Province of Saudi Arabia Against Some Antibiotic Resistance Human Pathogens
Ahmad Al-Ghamdi*, Mohd. Javed Ansari, Noori Al-Waili
(Bee Research Chair, Department Of Plant protection, King Saud University Riyadh, Saudi Arabia)
Propolis is a resinous substance collected by worker honeybees from various plant sources. The bees pack propolis on their hind legs, carry it to their colony, and use it as a sterilant in the hive. It is one of the oldest known medicines that are used even today in folk medicines. Although numerous researchers have been reported the antimicrobial activity of propolis from different countries and geographical regions but information about Saudi Arabian Propolis are still limited.
In this study, antimicrobial effects of propolis samples on Gram Negative Escherichia coli, Gram positive Staphylococcus aureus strain that are multiresistant to antibiotics and on fungal human pathogen Candida albicans were studied by using microbroth dilution Assay. Propolis extract were prepared with 70% ethyl alcohol at concentration of 1%, 0.75%, 0.50%, 0.25 % and 0.10% (v/v) in nutrient broth. Ethanol (70%) were used as a controls. At concentrations of 0.25 % ,(minimum inhibitory concentration) propolis extracts kill all of the microorganisms and 70 % alcohol has no effect on Pathogens.
In conclusion Saudi Arabian Propolis had a strong and dose dependent activity against most of the human pathogen and the results detected in this in vitro study provided evidence that propolis may be used as an antimicrobial agent against many HUMAN pathogen microorganisms.
*Corresponding author,E-mail:aalkhazim@gmail.com
Ahmad Al-Ghamdi*, Mohd. Javed Ansari, Noori Al-Waili(沙特阿拉伯)
(Bee Research Chair, Department Of Plant protection, King Saud University Riyadh, Saudi Arabia)
蜂胶是西方蜜蜂工蜂采集于多种植物的。蜜蜂将蜂胶装在后肢上,回蜂巢,将其作群的。蜂胶是至今仍在使用的最古老的民之一。然关于不同国家和地区的蜂胶抗菌活性研道很多,但是沙特阿拉伯蜂胶抗菌活性却道。本研究采用微量肉法,研究蜂胶多种抗生素的革氏阴性大杆菌和革氏阳性金黄色酿脓葡萄球菌菌株以及真菌性的人病原菌白色念珠菌的抗菌活性。在养液体培养基中加入70%乙醇的蜂胶提取液,使其度分别为0.10%、0.25%、 0.50%、0.75 % 和 1%(v/v),70%乙醇作为对。在最小抑菌度即0.25%蜂胶提取液,即可死所有的微生物,而70%乙醇病原菌无任何作用。因此,沙特阿拉伯蜂胶大部分人病原菌具有很的抗菌活性而且具有量依性。一体外研究得到的果也表明蜂胶或可以作为许多人病原菌的抗菌键词:抗菌活性;沙特阿拉伯蜂胶;人病原菌;微量肉

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