Cytotoxic Action of Brazilian Propolis In Vitro on Canine
Osteosarcoma Cells
Phytotherapy Research, Early View
Osteosarcoma (OSA) is a primary bone neoplasm frequently
diagnosed in dogs. The biology of OSA in pet dogs is identical to that of
pediatric patients, and it has been considered an excellent model in vivo to
study human OSA. Since the individual response to chemotherapy is unpredictable
and considering that propolis is a natural product with several biological
properties, this work evaluated the cytotoxic action of propolis on canine OSA
cells. The primary cell culture of canine OSA was obtained from the tumor of a
dog with OSA. Cell viability was assessed after incubation with propolis, 70%
ethanol (propolis solvent), and carboplatin after 6, 24, 48, and 72 h. Cell
viability was analyzed by the crystal violet method. Data showed that canine
OSA cells were sensitive to propolis in a dose- and time-dependent manner and
had a distinct morphology compared to control. Its solvent (70% ethanol) had no
effect on cell viability, suggesting that the cytotoxic action was exclusively
due to propolis. Our propolis sample exerted a cytotoxic effect on canine OSA
cells, and its introduction as a possible therapeutic agent in vivo could be
investigated, providing a new contribution to OSA treatment.
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