Ethanol Extract of Brazilian Red Propolis Induces Apoptosis in
Human Breast Cancer MCF-7 Cells Through Endoplasmic Reticulum Stress
J Agric Food Chem, 2012 Oct 15
Propolis, a natural product collected from plants by honey
bees, is commonly used in folk medicines. Endoplasmic reticulum (ER) stress is
known to induce apoptosis through the induction of CCAAT/enhancer-binding
protein homologous protein (CHOP). Here, we investigated whether ethanol
extracts of propolis and caffeic acid phenethyl ester (CAPE) induce apoptosis,
mitochondrial dysfunction and ER stress in human breast cancer MCF-7 cells and
human fibroblasts. Among several ethanol extracts of propolis and CAPE,
Brazilian red propolis (BRP) significantly reduced MCF-7 cell viability through
the induction of mitochondrial dysfunction, caspase-3 activity and DNA
fragmentation, but did not affect those of fibroblasts. Moreover, treatment
with BRP significantly induced CHOP expression in MCF-7 cells compared to
fibroblasts. Further, pretreatment with a chemical chaperone, 4-phenylbutyric
acid, suppressed BRP-triggered MCF-7 cell death. Overall, we revealed that an
ethanol extract of BRP induces MCF-7 cell apoptosis through, at least in part,
ER stress-related signaling.
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