Oct. 22-25, 2012
Acute Toxicological Test of Propolis
ZHUO Bi-rong, SHI Pei-ying, WU Zhen-hong*
(Bee Science College of Fujian Agriculture and Forestry University, State and Local Joint Engineering Laboratory of Natural Biotoxi, Fuzhou 350002, China )
This project used Fixed-dose procedure to study acute toxicity reaction of mice to ethanol extract of propolis. The result showed that mice, which have been lavaged with ethanol extract of propolis were all live during 0~14 days. In addition, the tested mice shown no-affected locomotor activity and had no abnormal symptoms. The mice showed no abnormal secretion in mouths, noses and ears. Also the mice showed other normal symptoms such as eyelid without prolapse, clear eye pupil, normal excretions, and smooth hair. Autopsy results showed no obvious abnormity characterization of the organs. The organs index has no obvious difference with control group (The max dose is 2000mg/kg).
In conclusion, mice had no acute toxicity reaction to ethanol extract of propolis. Since 2000mg/kg is 100 times of the human body recommended dose, people can also use propolis with this dose.
*Corresponding author, E-mail:firstname.lastname@example.org
采用固定剂量法研究蜂胶醇提物对正常小鼠的急性毒性反应，经过研究发现，对小鼠灌胃蜂胶醇提物 0~14 d 内，小鼠无死亡，且其自主活动无异常症状，口、鼻、耳等无异常分泌物，眼睑无下垂，眼瞳清澈，两便正常，毛发光滑，尸检各器官无明显异常表征，各器官指数与对照组相比无显著差异，最高的使用剂 量为2000 mg/kg；综上说明了蜂胶醇提取物对正常小鼠无严重的急性毒性反应。且因2000 mg/kg 大约是人体推荐服用剂量的100 倍，因此人体可放心使用。