The Synergistic Cytotoxic Effect Of Cisplatin and Honey Bee
Venom on Human Ovarian Cancer Cell Line A2780cp
J Venom Res, 2012;3:22-7
Ovarian cancer is considered to be one of the most important
causes of death among women. Cisplatin is one of the oldest chemotherapeutical
compounds used for treating ovarian cancer. Previous studies have shown the
inhibitory effects of bee venom on certain types of cancer.
The aim of the present study was to evaluate the cytotoxic
effect of bee venom alone and its synergistic cytological effects in
combination with cisplatin on ovarian cancerous cisplatin resistant A2780cp
cells. To investigate the cytotoxic effect of bee venom on A2780cp cells and
its synergetic effect with cisplatin, MTT assay, morphological examination, DNA
fragmentation assay, flowcytometric and immunocytochemical analysis were
performed. MTT assay revealed that 8µg/ml bee venom, 25mg/ml cisplatin and
4µg/ml bee venom/10mg/ml cisplatin cause an approximately 50% A2780cp cell
death after 24hr. Morphological and biochemical analysis indicated an apoptotic
type of cell death induced by bee venom and cisplatin, separately and in
combination. Immunocytochemistry demonstrated a reduction in the levels of the
Bcl2 protein.
Overall, our findings suggest that components of bee venom
may exert an anti-tumor effect on human ovarian cancer and that has the
potential for enhancing the cytotoxic effect of the antitumor agent cisplatin.
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