Evid Based Complement Alternat Med, Epub 2012 Dec 18
Caffeic acid phenethyl ester (CAPE), an active component
extracted from honeybee hives, exhibits anti-inflammatory and anticancer
activities. However, the molecular mechanism by which CAPE affects oral cancer
cell metastasis has yet to be elucidated.
In this study, we investigated the potential mechanisms
underlying the effects of CAPE on the invasive ability of SCC-9 oral cancer
cells. Results showed that CAPE attenuated SCC-9 cell migration and invasion at
noncytotoxic concentrations (0 μM to 40 μM). Western blot and gelatin
zymography analysis findings further indicated that CAPE downregulated matrix
metalloproteinase-2 (MMP-2) protein expression and inhibited its enzymatic
activity. CAPE exerted its inhibitory effects on MMP-2 expression and activity
by upregulating tissue inhibitor of metalloproteinase-2 (TIMP-2) and potently
decreased migration by reducing focal adhesion kinase (FAK) phosphorylation and
the activation of its downstream signaling molecules p38/MAPK and JNK.
These data indicate that CAPE could potentially be used as a
chemoagent to prevent oral cancer metastasis.
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