Comparison of the Antibacterial Efficacy of Manuka Honey
Against E.faecalis and E.coli - An In vitro Study
J Clin Diagn Res. 2014 Aug;8(8):ZC29-31
AIM:
To compare the antibacterial efficacy of Manuka honey
against E.faecalis and E.coli.
MATERIALS AND METHODS:
Escherichia coli (ATCC-25922) and Enterococcus faecalis
(ATCC-29212) were separately inoculated in the nutrient broth and incubated at
37(o)C for 24-48 hrs. Bacterial samples were kept in contact with each
disinfectant solution for varying intervals of time. Once the test time had
elapsed 10μL of the bacterial dilutions were plated on Mueller-Hinton agar and
incubated for 24-48 hrs at 37°C to estimate the density. Study of the disinfection
process with respect to time and Modeling was done.
RESULTS:
The mean value of the antimicrobial activity of Manuka honey
against E.coli and E.faecalis are 1.55 and 0.36 respectively and are relatively
higher. It shows that there is a significant difference among the various root
canal disinfectant groups against E. coli and E. feacalis. (p < 0.001)
CONCLUSION: Manuka honey is shown to be a potential root
canal disinfectant against gram positive and gram negative bacterial pathogens.
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