Accelerated wound healing and anti-inflammatory effects of
physically cross linked polyvinyl alcohol-chitosan hydrogel containing honey
bee venom in diabetic rats
Arch Pharm Res, 2013 Nov 30
Diabetes is one of the leading causes of impaired wound
healing. The objective of this study was to develop a bee venom-loaded wound
dressing with an enhanced healing and anti-inflammatory effects to be examined
in diabetic rats.
Different preparations of polyvinyl alcohol (PVA), chitosan
(Chit) hydrogel matrix-based wound dressing containing bee venom (BV) were
developed using freeze-thawing method. The mechanical properties such as gel
fraction, swelling ratio, tensile strength, percentage of elongation and
surface pH were determined. The pharmacological activities including wound
healing and anti-inflammatory effects in addition to primary skin irritation
and microbial penetration tests were evaluated. Moreover, hydroxyproline,
glutathione and IL-6 levels were measured in the wound tissues of diabetic
rats.
The bee venom-loaded wound dressing composed of 10 % PVA,
0.6 % Chit and 4 % BV was more swellable, flexible and elastic than other
formulations.
Pharmacologically, the bee venom-loaded wound dressing that
has the same pervious composition showed accelerated healing of wounds made in
diabetic rats compared to the control. Moreover, this bee venom-loaded wound
dressing exhibited anti-inflammatory effect that is comparable to that of
diclofenac gel, the standard anti-inflammatory drug.
Simultaneously, wound tissues covered with this preparation
displayed higher hydroxyproline and glutathione levels and lower IL-6 levels
compared to control.
Thus, the bee venom-loaded hydrogel composed of 10 % PVA, 0.6 % Chit and 4 % BV
is a promising wound dressing with excellent forming and enhanced wound healing
as well as anti-inflammatory activities.
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