Effect of Gelam Honey on the Oxidative Stress-Induced
Signaling Pathways in Pancreatic Hamster Cells
Int J Endocrinol, 2013;2013:367312
Background: Oxidative stress induced by reactive oxygen and
nitrogen species is critically involved in the impairment of β -cell function
during the development of diabetes.
Methods. HIT-T15 cells were cultured in 5% CO2 and then
preincubated with Gelam honey extracts (20, 40, 60, and 80 µg/mL) as well as
quercetin (20, 40, 60, and 80 µM), prior to stimulation by 20 and 50 mM of
glucose. Cell lysate was collected to determine the effect of honey extracts
and quercetin on the stress activated NF- κ B, MAPK pathways, and the Akt
(ser473) activated insulin signaling pathway.
Results. HIT-T15 cells cultured
under hyperglycemic conditions demonstrated insulin resistance with a
significant increase in the levels of MAPK, NF- κ B, and IRS-1 serine
phosphorylation (ser307); however, Akt expression and insulin contents are
significantly decreased. Pretreatment with quercetin and Gelam honey extract
improved insulin resistance and insulin content by reducing the expression of
MAPK, NF- κ B, and IRS-1 serine phosphorylation (ser307) and increasing the
expression of Akt significantly.
Conclusion. Gelam honey-induced differential
expression of MAPK, NF- κ B, IRS-1 (ser307), and Akt in HIT-T15 cells shows
that Gelam honey exerts protective effects against diabetes- and hyperglycemia-induced
oxidative stress by improving insulin content and insulin resistance.
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